Effects of biomechanical stress on chondrocyte ECM expressionWe presume that biomechanical stress could induce the matrix degradation and decrease the matrix synthesis. as pointed out above, Chondrocytes were encountered with pre optimized magnitudes of CTS; The expression levels of COL2A1 and aggrecan were certainly downregulated, Whereas the expression levels of MMP1 and MMP13 were increasingly upregulated, And ADAMTS5 expression was pretty much unaffected by CTS (Fig. 6a). concur, Chondrocytes were discolored with Toluidine blue and Alcian blue. the results showed that the chondrocytes stimulated with CTS included fewer ECM components than unstimulated cells (Fig. 6b). The ECM related proteins COL II and MMP13 were also examined by immunofluorescence staining. final results showed that the expression of COL II decreased, Whereas the period of MMP13 increased in chondrocytes after CTS (Fig. 7).
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