The gateway system (Invitrogen, Carlsbad, los angeles, australia) was required for subcloning genes into retroviral vectors. The destiny vectors, PCLXSN DEST but pDEST CMSCVpuro, Were crafted by inserting a modified cassette containing attR sites and ccdB (Invitrogen) concerned with the XhoI and BamHI sites of pCLXSN, And between the EcoRI and BglII sites of pCMSCVpuro, […]