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EIF5A rapidly translocates to the Golgi gear and is tyrosine sulfated in the trans Golgi for secretionIn general, Intracellular proteins that are ultimately secreted into the extracellular space are viewed to require an N terminal signal peptide (established secretory pathway). but bear in mind, Evidence has gained that some proteins, most notably cytokines and growth factors, Lacking an N fatal signal peptide can be secreted (Non classical secretory pathway)17,18. Most tyrosine sulfated proteins detected to date are secreted. These secretory healthy proteins are tyrosine sulfated in the trans Golgi and are then sorted into membrane vesicles, Which bring these proteins to the plasma membrane for secretion14,16. We confirmed the rapid pileup and the marked tyrosine sulfation of eIF5A in the trans Golgi (Using syntaxin 6 as any marker20); These events actually peaked at 2min after reoxygenation (Fig. 2f).
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