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2). man viruses were then grown in Madin Darby canine kidney (MDCK) Cells changed to overexpress Sia (AX4 cells17), And screened again for astounding to agglutinate Sia (additional Fig. 2). The adult control virus (chosen VN1203/PR8) With avian type receptor binding specificity agglutinated untreated TRBCs (Which possess both human and avian type receptors on the surface), But not TRBCs possessing predominantly human type receptors (Sia ancillary Table 1).
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