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Statue 3: results of Mg2+ binding on G GDP.(the particular) Overlay in 1H 15N TROSY spectra of u 2H, 15N, Ala, Ile1 [13CH3] G GDP in the activity (teal) And lack (black) using 20mM Mg2+. The Mg2+ amount dependence of the Phe191 signal is shown below. (j) Overlay together with the 1H 13C average nfl salary 1960 HMQC spectra of u 2H, 15N, Ala, Ile1 [13CH3] G GDP in the levels (ruddy) And dearth (white) pointing to 20mM Mg2+. The Mg2+ focus dependence of the Ile49 signal is shown below. (g) The intensity ratios of the two signals observed for Phe191 and Ile49 were plotted against the Mg2+ focus. The apparent dissociation constants worked out from the Phe191 and Ile49 signals were 3.1 not to mention 3.0mM, Respectively. (deborah) The Ala and Ile methyl groups with central chemical shift differences are mapped on the crystal structure of G GDP bound to Mg2+ (PDB i. d,recognition: 1BOF)34. Range are dyed pink. (e) 13C SQ CPMG dispersion studies of Ile49 and Ile222. The results in the employment of 5mM Mg2+ are coloured red, And those in the absence of Mg2+ are coloured black. The error bars represent the experimental errors calculated using the equation (16). The fitted parameters are summarized below. The dimensions were performed at 20C with a Bruker Avance 600 spectrometer. The 1H 13C HMQC spectra of the related residues are also shown.
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