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Culture of primary chondrocytes and experience of mechanical stressDonor chondrocytes were isolated as previously described41. The chondrocytes were at passage 2 and fibroblasts had been as control. We detect specific markers in both cells by immunofluorescence analysis. final results showed that the expression of Sox9 is high in our chondrocytes, While short of fibroblasts (second Figure 1A). the actual, reflection of S100A4 is low in our chondrocytes, While good for fibroblasts (supplementary Figure 1B). Chondrocytes (5105/well) Were grown on pronectin coated Bioflex six well traditions plates (Flexcell you can also use, Hillsborough, nc) so that it will 80% confluence. Cyclic tensile form (CTS) findings were performed using the FX 5000 Flexcell system (Flexcell overseas, McKeesport, pennsylvania). CTS was forced at 5%, 10%, in addition to 15% elongation (0.5Hz) for 24h42,43.
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