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The crystal shape of wild type G GDP (ref. 17) revealed that Asp150, situated on the E helix in the helical domain, Forms no direct human relationships with the bound GDP, due to this fact, The accelerated GDP dissociation seems to result from the structural differences in G GDP induced by the mutation. To gain structural insights into the mechanism of the GDP dissociation, We made NMR analyses for the wild type and the D150N mutant. simply because of the relatively large molecular weight of G GDP (41K), We adopted selective methyl labelling programs and applied methyl TROSY techniques18,19. We prepared a selectively labelled u 2H, Ile1, Leu2, Val2 [13CH3] G GDP bit of music, And compared the 1H 13C heteronuclear countless quantum (MQ) Coherence (HMQC) Spectra (Fig. 1c and second Fig. 2). incredibly, the chemical shift differences between the wild type and the D150N mutant were quite small, And the weighted average chemical shift distinctions were Fig. 1e).
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