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Amarlo Herrera Number Jersey To characterize further the receptor binding properties of the chosen variants, We used solid phase binding assays the place sialylglycopolymers were absorbed to plates, that had been then incubated with virus (Fig. 2a). A virus being the owner of the HA and NA genes of the seasonal human A/Kawasaki/173/2001 (H1N1; K173) Virus and the remainder genes from PR8 (K173/PR8) Served as a control virus with typical human type receptor specificity. in reality, K173/PR8 preferentially bound to Sia in contrast, VN1203/PR8 bound to only Sia As informed elsewhere6, 15, The Q226L/G228S strains led to increased binding to Sia Variants I202T/R220S, W153R/T160I, N169I/H184L/I217M and H130Q/K157E was best nfl card hobby box to get similar to VN1203/PR8 in their binding to glycans, even though that these mutants weakly agglutinated Sia (See supplementary Table 1). These viruses may have bound to glycans on TRBCs that were totally different from Sia used in this study. to spot the amino acid change(s) with regard to the conversion from Sia to Sia recognition in the E119G/V152I/N224K/Q226L virus HA, We tested the amino acid changes at positions 119, 152, 224 and 226 on their own and in various combinations. Solid phase binding assays demonstrated that the N224K/Q226L combination is critical for the shift from Sia to Sia recognition (Fig. 2b); Q226L in combination with V152I also conferred weak binding to
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